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LC-MS/MS Analysis Kits
KITS , CALIBRATORS & CONTROLS
LC-MS/MS Analysis Kits

Vitamin ADEK Serum LC-MS/MS APCI Analysis Kit

Vitamin ADEK Serum LC-MS/MS APCI Analysis Kit

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CE-IVD certified and suitable for any kind of LC-MS/MS instrument

The Zivak Vitamin A- D3-D2-E-K1 analysis kit can be used in combination with the Zivak-Multitasker as well as manually. Analysis run time for the manual version is 5 minutes. Due to the efficient and quite smart procedure of the Zivak-Multitasker, the automated version guarantees also to receive results in 1.7 minutes per test, including sample preparation-, sample injection- and analysis run time. Additionally, high accuracy is being rectified through NIST (National Institute of Standards and Technology) with an accuracy of 96%.

  • Vitamin D, commonly known as the “sunshine vitamin”, is essential for maintaining calcium metabolism and bone health. The major physiologic function of Vitamin D is to maintain the blood calcium and phosphorus levels within the normal range in order to maintain metabolic functions that are essential for most life processes, Vitamin D3 and Vitamin D2 are metabolized to 25-OH-Vitamin D3 and 25-OH-Vitamin D2 in the liver by the 25-OHase enzyme. The quantitation of these metabolites is widely used for determining of Vitamin D status in human. The Vitamin D status has a clinical significance in a variety of disorders which related to hormones. The measurements of the serum concentration of 25-OH-Vitamin D3 and 25-OH-Vitamin D2 have been used for differential diagnosis of hypocalcemia and hypercalcemic metabolic bone disorders.

The analysis of these metabolites is considered to be difficult. Some reasons of the     difficulties are below.

  • The low concentrations of 25-OH Vitamin D metabolites in serum. (Level of µg/L)
  • The low stability in the presence of light and heat.
  • The presence of other metabolites which have chemical similarity.
  • The large amount of other related neutral lipids (such as cholesterol)

  

  • The level of vitamin A in the plasma or serum is a reflection of the quantities of vitamin A and carotene ingested and absorbed by the intestine.

Vitamin A plays an essential role in the function of the retina (adaptation to dim light), is necessary for growth and differentiation of epithelial tissue, and is required for growth of bone, reproduction, and embryonic development. Together with certain carotenoids, vitamin A enhances immune function, reducing the consequences of some infectious diseases. Vitamin A in excess can be toxic.

 

 

 

Vitamin E contributes to the normal maintenance of bio membranes, the vascular system, and the nervous systems; and provides antioxidant protection for vitamin Deficiency of vitamin E in children leads to reversible motor and sensory neuropathies; this problem also has been suspected in adults.

Deficiencies of vitamins A and E may arise from poor nutrition or from intestinal malabsorption. People, especially children, at risk include those with bowel disease, pancreatic disease, chronic cholestasis, celiac disease, cystic fibrosis, and intestinal lymphangiectasia.

  • Vitamin K is a fat-soluble vitamin with important roles in blood coagulation factor synthesis, bone mineralization and vascular calcification. There are various forms of vitamin K. Vitamin K1 (phylloquinone) is synthesized by plants. At the Nutritious unit we assess tissue stores by measuring serum vitamin K1 levels, from which it is possible to identify suboptimal status before the appearance of symptoms. In addition to vitamin K1 we also measure undercarboxylated prothrombin (PIVKA-II) to provide additional insight into the nature and extent of the deficiency. PIVKA-II can also help investigate cases where vitamin K antagonists are involved or complications due to poor hepatic function. Screening for vitamin K deficiency is recommended in high-risk groups such as those with malnutrition, malabsorption (e.g. cystic fibrosis, short bowel, Crohn’s disease, biliary problems etc.) or those who may have impaired utilization of vitamin K e.g. liver disease.

Vitamin A-D3-D2-E-K1 Serum LC-MS/MS APCI Analysis Kit was developed for rapid, sensitive, and reliable quantitative detection of the Vitamin A-D3-D2-E-K1 concentrations in human serum samples and it gives results in 10 minutes with full automated Zivak MULTITASKER sample preparation and injection system. Main methods and procedures that have been selected are based on EN ISO 13485 and 98/79/EC.

LC-MS/MS parameters : contact for more details

•   This analysis kit can be shipped at room temperature.

•   Serum controls, calibrators and reagent 2 must be stored at 2-8 °C until reconstitution.

•   After arrival, mobile phases and reagent 1 must be stored at room temperature.

•   After reconstitution serum calibrators and controls must be stored at -20 °C.

•   All components are guaranteed until expiry date when stored at recommended temperatures and used as described in these instructions

Preparation of Calibrators and controls : contact for more details

Contact for more details : info@zivak.com

Zivak General Catalog

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