World First Walk Away Vitamin D2/D3 UHPLC Analyzer
Why we analyze 25-OH Vitamin D2 and D3 separately?
Circulating 25-hydroxyvitamin D (25-OH-D) is widely recognized as the best indicator of vitamin D status. In blood circulation the two major vitamin D metabolites are 25-OH Vitamin D3 and 25-OH Vitamin D2. 25-OH-D3 is mainly derived from vitamin D3 produced by sunlight in the skin, while 25-OH-D2 is derived from plants in the diet. There are currently two main types of methods used routinely for measuring 25-OH-D: methods based on chromatographic separation followed by non-immunological direct detection and competitive immunoassays. Most immunoassays depend on an antibody that can detect both 25-OH Vitamin D2 and 25-OH Vitamin D3 together and gives total results. But the proportion of 25-OHD2 detection is variable.
Two non-immunological methods for direct detection are currently available: high pressure liquid chromatography (HPLC) and liquid chromatography-tandem mass spectrometry (LC-MS/ MS). Chromatographic methods are able to measure 25-OH-D2 and 25-OH-D3 independently with excellent accuracy and sensitivity.
On the other hand, there is many scientific studies and papers showing inaccuracy measuring total Vitamin D with commercial immunoassays without separating and measuring Vitamin D2 and Vitamin D3 metabolites individually. It is recommended that any 25-OH-D assay in clinical laboratories should measure both 25-OH-D2 and 25-OH-D3 equally in order to report a total 25-OH-D value. The evaluated immunoassays measure total 25-OH-D (both 25-OH-D3 and 25-OH-D2), but the cross-reaction with 25-OH-D2 and 25-OH-D3 differs from 52 to 100% in different methods.
Walk Away Analyzer
HPLC and LC-MS/MS methods are accepted as Gold Method for separate analysis of 25-OH metabolites of Vitamin D. Usage of these chromatographic methods are being increased significantly on clinical laboratories recent years. The major disadvantage is the time-consuming and laborious extraction procedure that limits the number of samples that can be processed per day.
Many of these laboratories cannot use their chromatography systems effectively due to these systems required qualified staff, complex sample preparation steps, high kit prices/ operation costs and no availability of walk away systems.
VD-200 enables to analyze Vitamin D2/D3 samples fully automated – without any human intervention- from primer sample tube. By the way, the low throughput in chromatography analysis will not be a problem for the laboratories.
Due to large number of Vitamin D2/D3 samples, many laboratories cannot run all other LC-MS/MS specific analysis on their LC-MS/MS systems effectively. VD-200 is especially designed the for the laboratories to provide them a cost- effective solution with a fully automated system and make customer’s expensive LC-MS/MS systems free for specific tests which have to be run on LC-MS/MS system.
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